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CD97 Antibody

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CD97 Antibody

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CD97 Antibody

 
  • 货号:
    HYK-200293
  • 规格:
     
  • 图片:
    • IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • IHC image of CSB-RA004972A0HU diluted at 1:100 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of Hela cells with CSB-RA004972A0HU at 1:87.5,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

产品详情

  • Uniprot No.:
    P48960
  • 基因名:
    CD97
  • 别名:
    ADGRE5 antibody; Adhesion G protein-coupled receptor E5 antibody; CD 97 antibody; CD_antigen=CD97 antibody; CD97 antibody; CD97 antigen antibody; CD97 antigen subunit beta antibody; CD97 molecule antibody; CD97_HUMAN antibody; Leukocyte antigen CD97 antibody; Seven span transmembrane protein antibody; Seven transmembrane helix receptor antibody; Seven transmembrane heterodimeric receptor associated with inflammation antibody; TM7LN 1 antibody; TM7LN1 antibody
  • 免疫原:
    A synthesized peptide
  • 免疫原种属:
    Homo sapiens (Human)
  • 标记方式:
    Non-conjugated
  • 克隆类型:
    Monoclonal
  • 抗体亚型:
    Rabbit IgG
  • 纯化方式:
    Affinity-chromatography
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液:
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 产品提供形式:
    Liquid
  • 应用范围:
    ELISA, IHC, IF
  • 推荐稀释比:
    Application Recommended Dilution
    IHC 1:50-1:500
    IF 1:30-1:200
  • Protocols:
    ELISA Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
  • 储存条件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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