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【文献标题】Interference of ATP-Binding Cassette Transporter by Functional Nano-Delivery System for High Efficiency Management of Insecticide-Resistant Nilaparvata lugens
【作者】Chengshuai He ，Hejun Ren ，Shuo Zhang，et.al
【作者单位】南京农业大学（Nanjing Agricultural University）
【文献中引用产品】
昆虫三磷酸腺苷（ATP）ELISA试剂盒
昆虫ABC转运蛋白（ABC）ELISA试剂盒
【DOI】https://doi.org/10.1002/advs.76866
【影响因子(IF)】14.1
【出版期刊】《Advanced Science》
【产品原文引用】
The effect of ZIF-90 on ATP levels in vitro was assessed in Sf9 cells. Briefly, Sf9 cells were seeded in 6-well plates and treated with 100 mg L 1 ZIF-90. After 24 h of incubation, the cells were collected by centrifugation at 1, 000 rpm for 5 min. The cell pellet was thoroughly lysed on ice using 250 μL of RIPA lysis buffer (Solarbio, Beijing, China) containing 1 mM freshly prepared PMSF. The lysates were subsequently centrifuged at14,000 × g for 5 min at 4?C to obtain supernatants for ATP measurement. ATP concentrations were determined using an Insect ATP ELISA kit (Shanghai Hengyuan Biological Technology Co., Ltd., Shanghai, China) according to the manufacturer’s instructions.
The treatment of N. lugens was performed as described in the preceding ATP measurement section. ABC transporter levels were quantified using a commercial insect ABC transporter ELISA kit (Shanghai Hengyuan Biological Technology Co., Ltd., Shanghai,China) based on the double-antibody sandwich method, according to the manufacturer’sinstructions.
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